STAT3 Transcription Factor Assay kit -
The CHEMICON® Non-Radioactive STAT3 Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with significantly greater sensitivity than conventional EMSA assays. The entire assay takes less than 4 hours to complete with minimal hands-on time. The versatile set up allows for a flexible assay design and because the binding reaction occurs in solution, various parameters can be optimized such as probe titration, competitive oligonucleotide concentration, or treatment conditions. Application:STAT proteins, which serve dual roles as Signal Transducers and Activators of Transcription (STAT), are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors (1,2). The highly responsive nature of STATs lies in their ability to increase, in a matter of minutes, the transcriptional activity of various genes (1). A large body of evidence suggests that STAT signaling may play critical roles in cancer formation and progression in addition to the immune response (4). In contrast to STAT2 and STAT4, which are only activated by a small number of molecules, STAT1, -3, -5A, and -5B are activated by many different ligands raising the question of how these transcription factors participate in specific biological responses (3). STAT1, -3, -5A, and -5B are all between 750 and 795 amino acids long and are phosphorylated at a single tyrosine that is necessary for STAT activation (3). Each of the STATs has a crucial function in mice. STAT1-/- mice have no innate response to either viral or bacterial infections, presumably because of the need for the IFN response in the first stages of defense. STAT3 is the main mediator of interleukin 6 (IL-6)-type cytokine signaling and knockouts are embryonically lethal. When STAT3 is absent, cells exhibit phenotypes including impaired apoptosis, loss of negative feedback regulation, impaired cell migration and wound healing, and cell death. STAT5A and STAT5B play critical roles in milk production. Method:Complete Transcription Factor Assay Species: Storage:-20°C