Phosphatidylcholine Assay Kit - 1 Kit

In this enzyme coupled assay, phosphatidylcholine is cleaved by Phospholipase D to produce choline and phosphatidic acid. Choline is oxidized by choline oxidase to betain producing hydrogen peroxide. DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is measured by absorbance at 595 nm1. Application: Method: Species: Storage:-20° C